Journal: The Journal of Advanced Prosthodontics
Article Title: Effect of microgrooves and fibronectin conjugation on the osteoblast marker gene expression and differentiation
doi: 10.4047/jap.2015.7.6.496
Figure Lengend Snippet: The relative fold change osteoblast-marker-gene expression in human bone marrow-derived mesenchymal stem cells (MSCs) after 2-day confluence and 14 days of osteogenic culture on titanium substrata with specific surface topographies and fibronectin conjugation by quantitative real-time PCR. Note that the results are presented as a ratio to the mRNA expression levels of the reference GAPDH gene, followed by a standardization of the NE0's Ct (threshold cycle) expression as 1. One-way ANOVA (n = 5). *** : significant difference ( P < .001).
Article Snippet: Human plasma fibronectin (human pFN) (Sigma-Aldrich) was conjugated on the surface of the silanized Ti substrates in a 10 µg/mL concentration and maintained for 24 hours at 4℃.
Techniques: Marker, Expressing, Derivative Assay, Conjugation Assay, Real-time Polymerase Chain Reaction